40 Years of Horticulture Education in Cluj-Napoca

Dear participants, authors, guests and members of committees. We would like to inform you that we have already published the two issue (Volume 45 and 46, Issues 2, 2017 and 1, 2018) of Notulae Botanicae Horti Agrobotanici Cluj-Napoca journal (http://www.notulaebotanicae.ro/index.php/nbha/index), containing contributions accepted after peer review process. You can revise these papers, and see content journal on-line. 
Will be a great pleasure to warmly welcome you to this special event, here in Cluj-Napoca!


“40 Years of Horticulture Education in Cluj-Napoca” http://conference.shst.ro

Tocopherol Content in Vegetable Oils Using a Rapid HPLC Fluorescence Detection Method

A quick and direct method based on reversed phase high performance liquid chromatography with fluorescence detector for measuring tocopherols (α , β + γ and δ) has been developed. Oils are diluted in methanol: hexane: tetrahydrofuran (neither previous extraction of tocopherols nor saponification procedure are required) and after being vortexed and centrifuged, an aliquot of the overlay was injected directly into an Alltima C 18 column. Acetonitrile and methanol (50: 50) mixture was used as a mobile phase with a flow rate of 1 mL min-1. Quantification of tocopherols was performed by fluorescence detector at 290 nm excitation wavelength and 325 nm emission wavelength. Tocopherols were separated at 25°C in less than 10 min after injection. The method has good limit of detection (9 ng g-1 for α-tocopherol and 8 ng g-1 for β-, γ- and δ- tocopherols) and reproducibility (CV< 2.9 %). This method can be used to assess the influence of genetic modification of oil seeds on the distribution of tocopherols or the effect of tocopherols on the oxidative stability of edible plant oils.

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Antifungal Activity of Plant Essential Oils Against Botrytis cinerea, Penicillium italicum and Penicillium digitatum

Plant essential oils have the potential to replace the synthetic fungicides in the management of postharvest diseases of fruit and vegetables.The aim of this study was to access the in vitro and in vivo activity of essential oil obtained from oregano (Origanum vulgare L. ssp. hirtum), thyme (Thymus vulgaris L.) and lemon (Citrus limon L.) plants, against some important postharvest pathogens (Botrytis cinerea, Penicillium italicum and P. digitatum). In vitro experiments indicated that P. italicum did not show any mycelium growth in presence of thyme essential oils at concentration of 0.13 μl/ml. Moreover, B. cinerea did not show any mycelium growth in presence of lemon and oregano essential oils at concentration of 17 μl/ml and 0.02 μl/ml, respectively. Moreover, the essential oils from three species were effective in reducing the spore germination. The in vivo experiments confirmed the strong efficacy shown in vitro by essential oils. These oregano and lemon oils were very effective in controlling disease severity of infected fruit by B. cinera in tomatoes, strawberries and cucumbers. In tomatoes, grey mould due to B. cinerea was completed inhibited by oregano essential oils at 0.30 μl/ml. Moreover, lemon essential oils induced a significant reduction of grey mould disease severity. In strawberries, grey mould was completed inhibited by lemon essential oils at 0.05 μl/ml. In addition, lemon essential oils at 0.05 μl/ml showed 39% reduction of infected cucumber fruits by B. cinerea. These results indicate that essential oils after suitable formulation could be used for the control of postharvest diseases caused by Botrytis and Penicillium pathogens.

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